Real-time PCR applications in clinical research and diagnostics

Polymerase chain reaction (PCR) has enabled enormous progress in the field of molecular biology in the last twenty-five years. It became popular due to its high sensitivity and specificity and was introduced to numerous scientific fields. Today many different variants of PCR exist, with real-time PCR being the most common. This method is being successfully used at our institution for a variety of different applications, among which gene expression analyzes and development of protocols for clinical diagnostics currently dominate. But the extreme flexibility and customizability of real-time PCR, coupled with our team’s expertise, ensures efficient application of this technique to various new research projects

Defect detection capability of pulsed transient thermography

Abstract In this work, investigations have been conducted on a novel technique for enhancing thermographic data, which is known as thermographic signal reconstruction, to assess its defect detection limitation on CFRP composite. For defect greater than 4mm diameter, results have indicated a 60% improvement in detectability

Intoxicación por cianuro posterior a infusión prolongada de Nitroprusiato de sodio: a propósito de un caso

El nitroprusiato de sodio fue aprobado desde 1974 como medicamento para las crisis hipertensivas. Desde entonces existe controversia sobre el potencial tóxico del mismo. La toxicidad por cianuro es una complicación poco común del nitroprusiato de sodio, que puede ser difícil de diagnosticar en pacientes críticamente enfermos. Dentro de los factores de riesgo está la utilización de infusiones prolongadas y dosis altas, por lo que se sugiere que la dosis no exceda 2 μg/kg/min. A continuación, se reporta el caso de un paciente de 49 años que ingresa a un servicio de urgencias después de un síndrome cerebrovascular y una emergencia hipertensiva que requirió infusión de nitroprusiato de sodio, seguida de signos y síntomas, los cuales sugirieron toxicidad. Entre dichos síntomas predominaban los cambios neurológicos, por lo cual fue manejado con hiposulfito de sodio y hubo una buena respuesta. El objetivo de este reporte de caso es sensibilizar al personal de salud sobre la necesidad de un uso adecuado del nitroprusiato y sobre la importancia de sospechar toxicidad en paciente con factores de riesgo

Aesthetic Performance of Wayang Wong Millennial

This article was completed in the context of the publication of our research on the Bali Innovative Wayang Wong. The purpose of writing this article is to analyse the aesthetic elements of Millennial Wayang Wong Performing Arts as a Balinese innovative Wayang Wong. In general, the aesthetic structure of the traditional Balinese Wayang Wong performance is performed by old and senior dancers. However, the aesthetic of the Wayang Wong Millennial structure was staged by involving junior dancers who were still children. This forms the question: What is the form of the Wayang Wong Millennial performing arts? This research was completed using qualitative methods. All data was collected with the aid of a photo camera and interview guidelines. Primary research was data obtained through observation and interviews with informants. While library research is used to optimise data acquisition. All data was analysed using aesthetic theory and symbol theory. The results showed that Wayang Wong Millennial performing arts are Balinese innovative Wayang Wong performing arts. The form of the Wayang Wong Millennial performance is classified as a colossal Balinese Wayang Wong performance art by involving children as Wayang Wong dancers. If we look at dance property, fashion make-up, choreography, Wayang stories, lighting and show duration, it can be seen that the Wayang Wong Millennial performance has its own aesthetic structure. Keywords: Aesthetics, Performing Arts, Wayang Wong, Millennial